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ARTIGO ORIGINAL

Micobactérias atípicas em biopróteses: causa potencial de endocardite com culturas negativas

Ivan S. J Casagrande; José Lucciola; Cláudio A Salles

DOI: 10.1590/S0102-76381986000100006

RESUMO

O crescimento de micobactérias atípicas foi observado em estudos experimentais in vitro de fragmentos de válvulas aórticas porcinas após tanning em solução de glutaraldeído a 0,625%. Foram incubados 100 fragmentos de valvas distintas em 4 meios de cultura diferentes, em um total de 400 culturas, utilizando-se os meios de Thioglycollate, Brain-Heart-Infusion, Sabouraud-Dextrose liqüido e Micosel, que permaneceram estéreis por 72 horas. Prolongando-se o tempo de incubação e utilizando-se técnicas especiais de repique e meios especiais para as subculturas, verificou-se o crescimento de micobactérias atípicas a partir do 5? dia, em 23% das amostras, a partir de culturas nos meios de Thioglycollate e Sabouraud, identificados laboratorialmente como Mycobacterium chelonei. Testes posteriores demonstraram resistência desta micobactéria à ação bactericida do glutaraldeído e sua eliminação completa ocorreu após a ação do formaldeído associado ao surfactante.

ABSTRACT

The growth of atypical mycobacteria was observed in experimental studies in vitro of porcine aortic valve (aortic wall coupon) after tanning in glutaraldehyde solution at 0.625%. One hundred aortic wall coupons from different valves were incubated in 4 different culture media in a total of 400 cultures, employing the Thioglycollate, Brain-Heart-Infusion, liquid Sabouraud-Dextrose and Micosel, which did not become positive for 72 hours. The growth of atypical mycobacteria was recognized in 23% of the samples from the Thioglycollate and Sabouraud media after the 5th day utilizing appropriate subculturing techniques and special culture media. The atypical mycobacteria were identifyed as the Mycobacterium chelonei, which was demonstrated to be resistant to glutaraldehyde and it was completely eliminated by the formaldehyde in association with the surfactant.
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